Abcam, ab250439, Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MRRF antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14140(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250439 is the carrier-free version of
ab181223
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRRF also known as Mitochondrial Ribosome Recycling Factor is a protein involved mechanically in the process of ribosome recycling after mRNA translation. It facilitates the disassembly of ribosomal subunits preparing them for a new cycle of protein synthesis. MRRF has a molecular mass of approximately 29 kDa. The protein is localized in the mitochondria which is where it performs its function in mitochondrial ribosome recycling.
Biological function summary
MRRF plays a significant role in mitochondrial translation by promoting the release of ribosomes from mRNA enabling further rounds of translation. It functions as part of the mitochondrial translation machinery and is involved in the maintenance of mitochondrial protein synthesis efficiency. The interaction with other mitochondrial translation factors allows MRRF to efficiently mediate the disassembly of post-termination ribosomal complexes.
Pathways
MRRF is critically involved in the mitochondrial translation process a subset of the broader protein synthesis pathway within the cell. It actively participates in the recycling phase of mitochondrial translation ensuring the effective reuse of ribosomal components. MRRF interacts closely with other proteins such as mtEFG1 (Mitochondrial Elongation Factor G1) to facilitate ribosome recycling and promote cellular energy production linking mitochondrial translation to oxidative phosphorylation.
Dysfunction or mutations of MRRF have associations with mitochondrial diseases which often result from impaired protein synthesis in mitochondria. Such conditions can include mitochondrial encephalomyopathy and other neuromuscular disorders. MRRF may also relate to proteins like MFN2 (Mitofusin 2) which are important in maintaining mitochondrial network stability and function; disruptions in these proteins can compound to exacerbate mitochondrial dysfunction-related disorders.