Abcam, ab250441, Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal E1 Ubiquitin Activating Enzyme 1/UBA1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14204(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Human, Mouse,
Applications:Flow Cyt (Intra), ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250441 is the carrier-free version of
ab181225
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The E1 Ubiquitin Activating Enzyme 1 also known as UBA1 performs the initial step in the ubiquitination process. This enzyme activates ubiquitin a small protein that tags other proteins for degradation by the proteasome. UBA1 binds to ubiquitin in an ATP-dependent manner facilitating its transfer to E2 ubiquitin-conjugating enzymes. UBA1 has a molecular mass of approximately 117 kDa and shows expression in various tissues particularly in actively dividing cells.
Biological function summary
The enzyme plays an essential role in protein homeostasis by initiating the ubiquitin-proteasome system. UBA1 actively contributes to cellular regulation by targeting damaged or misfolded proteins for degradation thereby maintaining protein quality control. The enzyme is part of a complex alongside other proteins involved in the ubiquitination pathway which include E2 enzymes and E3 ubiquitin ligases that work together to achieve selective protein ubiquitination.
Pathways
E1 Ubiquitin Activating Enzyme 1 functions centrally in the ubiquitin-proteasome pathway a major mechanism governing protein turnover. It cooperates with other enzymes such as E2 and E3 to form a cascade that ensures substrate specificity and timely degradation. The enzyme also connects to the NF-kB signaling pathway where it regulates the stability of inhibitor kB proteins influencing immune responses and inflammation processes.
Mutations or dysregulation of UBA1 contribute to spinal muscular atrophy (SMA) and X-linked spinal muscular atrophy (XL-SMA). These conditions involve motor neuron degeneration linked to the impaired ubiquitin-proteasome function. Additionally alterations in UBA1 activity can impact B-cell-activation through the NF-kB pathway possibly leading to autoimmune disorders. Understanding the interactions of UBA1 with associated proteins provides insight into potential therapeutic targets for treating these diseases.