Abcam, ab250466, Anti-OP-2 antibody [EPR13357] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal OP-2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13357,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody detects dominant ~35kDa extra band in some samples, e.g. WI-38 and SK-OV-3.

Product details:
ab250466 is the carrier-free version of
ab181253
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OP-2 also known as "Op protein" or "Anti-Op" is a well-characterized protein with a mass of approximately 45 kDa. This protein is widely expressed in various tissues including the brain liver and spleen. OP-2 has a significant role in several physiological processes through its mechanical functions. As a molecular component it engages in protein interactions that influence cellular communication and signal transduction. Researchers have devoted considerable attention to understanding the complete range of its interactions.
Biological function summary
The protein participates in essential regulatory networks and modulates cellular responses to external stimuli. It often acts as a part of multi-protein complexes that are critical for its function. These complexes facilitate the protein's ability to engage in cell cycle regulation and apoptosis. Additionally OP-2 influences the stability and degradation of certain denatured proteins which emphasizes its role in maintaining cellular homeostasis. The protein's significance extends to acting as a mediator in immune responses.
Pathways
OP-2 plays important roles in both the MAPK/ERK and NF-kB signaling pathways. These pathways are integral to the regulation of cell proliferation differentiation and survival. In the MAPK/ERK pathway OP-2 interacts with a variety of kinases and phosphatases impacting their activity and consequently affecting transcription factors such as c-Myc and Fos. Meanwhile within the NF-kB pathway OP-2 can modulate the activity of IKK-γ which is an important regulatory protein controlling NF-kB activation. Through these interactions OP-2 influences cellular responses to stress and inflammation.
OP-2 shows a strong connection to certain cancers and autoimmune diseases. For instance aberrations in OP-2 function or expression levels have been associated with breast cancer where it affects tumor growth and metastasis. Researchers have also found links between OP-2 and multiple sclerosis a chronic autoimmune condition suggesting its involvement in immune regulation. Other proteins such as TNF-α often intersect with OP-2 in these diseases contributing to disease pathology. Understanding these associations helps in identifying potential therapeutic targets for intervention.