Abcam, ab250521, Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ETV2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5229(3),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250521 is the carrier-free version of
ab181847
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ETV2 also known as ETS variant transcription factor 2 functions as a transcription factor important in the regulation of gene expression involved with endothelial lineage differentiation. This protein has a mass of approximately 38 kDa. ETV2 is mainly expressed in embryonic vascular endothelial cells as well as in adult tissues like the heart and lungs. Some studies also refer to it by the alternate name ER71. High expression levels in these key areas support its critical roles in vasculature development and function.
Biological function summary
ETV2 regulates endothelial cell development and vasculogenesis. It acts as an important switch for endothelial cell fate essentially directing stem cells towards an endothelial lineage. ETV2 often operates within a larger protein complex interacting with other transcription factors necessary for vascular differentiation. Its role indicates a pivotal function in blood vessel formation and remodeling greatly influencing vascular health.
Pathways
ETV2 is a part of the VEGF (vascular endothelial growth factor) signaling pathway and the NOTCH signaling pathway. In the VEGF pathway ETV2 directly influences genes responsible for angiogenesis the process of forming new blood vessels. The NOTCH pathway involves ETV2 in the differentiation and proliferation of endothelial cells. ETV2 interacts with proteins such as VEGFA and NOTCH1 both significant contributors to vascular biology and embryonic development.
ETV2 relates to cardiovascular diseases and certain cancers. Dysregulation of ETV2 expression can contribute to impaired blood vessel formation playing a part in conditions like atherosclerosis and peripheral artery disease. The link between ETV2 and cancers such as breast cancer reveals its involvement in tumor angiogenesis as tumors harness ETV2 to recruit blood vessels promoting their growth. ETV2 also connects to the protein HIF1A which plays a critical role in cancer angiogenesis under low oxygen conditions.