Abcam, ab250582, Anti-REA antibody [EPR14523] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal REA antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Human, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14523,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250582 is the carrier-free version of
ab182139
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
REA also known as repressor of estrogen receptor activity is a protein that plays a role in estrogen receptor signaling pathways. REA has a molecular mass of approximately 69 kDa. It expresses widely in various tissues including the heart brain and skeletal muscle. This protein interacts with a number of transcriptional regulators to modulate gene expression. "Antirea" and "Protin rea" sometimes refer to tools used to study its function or its expression in research contexts.
Biological function summary
REA acts as a co-regulator in estrogen receptor complexes influencing the transcriptional activity of these receptors. It binds with estrogen receptors to either suppress or enhance their activity based on the cellular context and signaling molecules involved. Through this modulation REA contributes to the regulation of genes involved in cell growth differentiation and survival. Its ability to interact with other proteins such as coactivators and corepressors positions REA as an important player in modulating cellular responses to estrogen.
Pathways
REA is embedded in the estrogen signaling pathway which is critical for the regulation of reproductive tissues and certain non-reproductive tissues. It interacts with proteins like steroid receptor coactivator-3 (SRC-3) and nuclear receptor corepressor (NCoR) to influence pathway dynamics. Additionally REA connects with the MAPK signaling pathway impacting cell proliferation and survival by interacting with proteins such as MAPK and ERK2. These interactions place REA as a central node in hormone signaling networks within the cell.
The alterations in REA function have links to breast cancer and endometriosis. In breast cancer abnormal REA levels can disrupt estrogen signaling affecting tumor growth and progression. Coregulators like SRC-3 further modulate the disease outcome through their interaction with REA. In the case of endometriosis REA influences estrogen-driven processes contributing to the pathology of the disorder. Understanding REA's interactions offers insights into therapeutic targets and potential treatment strategies for these conditions.