Abcam, ab250598, Anti-DDX21 antibody [EPR14495] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal DDX21 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14495,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250598 is the carrier-free version of
ab182156
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDX21 also known as RH-II/Gu is a member of the DEAD-box RNA helicase family. It weighs approximately 87 kDa and is expressed prominently in the nucleus. DEAD-box proteins are known for unwinding RNA secondary structures essential for various cellular processes. DDX21 plays an important role in ribosome biogenesis and transcriptional regulation. Its nuclear localization is critical for its function and it is detected in various cell types indicating a broad role in cellular mechanisms.
Biological function summary
DDX21 is involved in multiple fundamental cellular processes beyond just transcription and ribosomal RNA processing. It participates in the synthesis of rRNA and plays an active role in the regulation of ribosome assembly. Additionally DDX21 interacts with other members of the DEAD-box family forming a complex which is necessary for efficient RNA processing and modification. Its activity has influence over protein synthesis by regulating the availability of ribosomal components.
Pathways
DDX21 fits into the larger RNA processing and ribosome biogenesis pathways. It interacts with proteins like nucleolin and fibrillarin contributing to the assembly and maturation of ribosomes. In the ribosome biogenesis pathway DDX21 controls the processing of pre-rRNA together with other ribosomal proteins ensuring proper formation of functional ribosomes. DDX21 also participates in the stress response pathways indicating its integral role in adapting to cellular stress conditions.
DDX21's function is implicated in cancer and viral infections. High levels of DDX21 have been associated with tumorigenesis connecting it with oncogenic pathways. It interacts with proteins like p53 in the regulation of cellular growth and apoptosis. Additionally DDX21 plays a part in the replication of certain viruses suggesting a role in the viral life cycle and potential as a therapeutic target. Understanding DDX21’s involvement in these conditions may offer insights into novel diagnostic and treatment strategies.