Abcam, ab250721, Anti-EMC4 antibody [EPR15081] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal EMC4 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15081,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250721 is the carrier-free version of
ab184162
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EMC4 also known as Endoplasmic Reticulum Membrane Protein Complex Subunit 4 is a protein involved in the insertion of membrane proteins into the endoplasmic reticulum (ER). It has a molecular mass of approximately 29 kDa and is expressed widespread in various tissues. EMC4 is part of the EMC complex a multi-subunit complex in the ER contributing to the processes of protein folding and assembly in the cell's secretory pathway.
Biological function summary
The EMC4 protein functions as a chaperone assisting in the proper folding and integration of polytopic proteins. It is a part of the EMC complex that works as a stabilizing agent for nascent polypeptides preventing misfolding and facilitating protein maturation. This complex plays a role in maintaining cellular homeostasis and challenges the ER stress response ensuring that proteins reach their functional conformation.
Pathways
EMC4 has a critical role in the protein secretory pathway and ER-associated degradation (ERAD) pathway. The EMC complex interacts with signal recognition particles and the translocon complex supporting the translocation and integration of proteins into the ER membrane. This involvement is key to processing newly synthesized proteins correctly collaborating with proteins like Sec61 which form part of the translocon complex.
Anomalies in EMC4 expression or function have connections to neurodegenerative diseases and congenital disorders of glycosylation. Altered EMC4 function can disrupt ER homeostasis contributing to neurodegenerative conditions like Alzheimer's disease where it relates to proteins such as amyloid precursor protein (APP). Furthermore EMC4 mutations may lead to glycosylation defects impacting the proper functioning of proteins involved in cellular communication and structure.