Abcam, ab250723, Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TMEM43 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15378(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250723 is the carrier-free version of
ab184164
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM43 also known as LUMA is a transmembrane protein with a mass of approximately 47 kDa. This protein localizes mainly to the nuclear envelope where it plays a structural role in maintaining nuclear integrity. It is widely expressed in various tissues especially in cardiac and skeletal muscle cells. Through its location and structure TMEM43 contributes to the physical and functional stability of the nuclear envelope interacting with other nuclear envelope components.
Biological function summary
Researchers recognize TMEM43 as an important player in the integrity of cellular architectures maintaining nuclear shape and mechanical stability. It forms part of a larger protein complex within the nuclear envelope referred to as the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. The interactions between TMEM43 and other LINC complex proteins contribute to its role in cellular mechanical transduction and nuclear-cytoskeletal coupling essential for cellular responses.
Pathways
TMEM43 plays a significant role in pathways related to nuclear-cytoskeletal interaction and mechanotransduction. It associates with the LINC complex proteins like Emerin and Lamin A/C facilitating the integration of signals from the cytoskeleton to the nucleus. This involvement places TMEM43 within the context of mechanosensitive pathways important for cellular responses to mechanical stimuli which are relevant in tissue development and homeostasis.
TMEM43 links closely to certain cardiac conditions and muscular disorders. Mutations in this protein are known to cause arrhythmogenic right ventricular cardiomyopathy (ARVC) a life-threatening heart disease characterized by fibro-fatty degeneration of the right ventricle. This connection also implicates proteins like Desmoplakin and Plakophilin-2 in ARVC as they form part of the complex that interacts with TMEM43. Additionally TMEM43's dysfunction may relate to muscular disorders where altered interactions with members of the nuclear envelope proteins contribute to muscle weakness and degeneration.