Abcam, ab250726, Anti-Germinal Center Kinase/GCK antibody [EPR14304] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Germinal Center Kinase/GCK antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14304,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250726 is the carrier-free version of
ab184169
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Germinal Center Kinase (GCK) also known as GCK kinase or GCK is part of the STE20 family of serine/threonine protein kinases. It has a mass of approximately 72 kDa. GCK mainly expresses in lymphoid tissues and is important for signaling processes. Its activity involves phosphorylating downstream substrates which helps regulate cell signaling pathways associated with cell survival and apoptosis.
Biological function summary
This kinase plays a significant role in the body's immune response by participating in signal transduction pathways that govern germinal center formation and function. GCK operates as a kinase that is sometimes part of larger protein complexes which allows it to have diverse biological roles. Through various signaling cascades it influences cellular activities that are important for immune system function including B-cell development and antibody production.
Pathways
GCK integrates itself into major signaling routes such as the MAPK and JNK pathways. These pathways control a range of cellular processes including proliferation differentiation and stress responses. GCK interacts with other proteins like MEKK1 and 14-3-3 which facilitate its role in these pathways. Such interactions enable the regulation of important cellular functions by modifying target proteins through phosphorylation.
Dysregulated GCK activity has implications in various conditions such as autoimmune diseases and certain types of cancer. Abnormal GCK function may alter immune cell regulation which can result in autoimmune phenomena. Additionally modified GCK signaling impacts cancer progression where its interaction with tumor suppressor proteins like p53 may influence tumorigenesis and tumor survival mechanisms.