Abcam, ab250752, Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal EIF2S2/EIF2B antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15833(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, IP, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250752 is the carrier-free version of
ab184549
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF2S2 also known as eukaryotic translation initiation factor 2 subunit 2 and EIF2B part of eukaryotic translation initiation factor 2B are key players in the initiation of protein synthesis. EIF2S2 is a 38.3 kDa protein and is expressed in various tissues including the brain liver and muscle. These factors form a complex that facilitates the exchange of GDP for GTP on the EIF2 complex important for the delivery of initiator tRNA to the ribosome.
Biological function summary
EIF2S2 and EIF2B are part of the eukaryotic translation initiation factor complex which plays a significant role in protein synthesis regulation under stress conditions. This complex senses cellular stress and decreases global protein synthesis by controlling the phosphorylation status of EIF2 allowing the selective translation of stress response proteins. This mechanism helps cells to conserve resources during unfavorable conditions while still expressing proteins necessary for survival.
Pathways
EIF2S2/EIF2B orchestrates the integrated stress response (ISR) pathway and the unfolded protein response (UPR) pathway. These pathways sense disruptions in protein folding and energy stress respectively. The phosphorylation of EIF2α related to these pathways is an important regulatory step that reduces general protein synthesis and channels the cellular machinery towards resolving stress.
Defects in EIF2S2 and EIF2B contribute to disorders such as Vanishing White Matter disease and some cancers. Vanishing White Matter disease linked to mutations in EIF2B affects brain white matter and involves the regulation of stress responses through proteins like EIF2α. In certain cancers abnormalities in the EIF2 pathway can affect cell survival and proliferation linking the protein to tumorigenesis and cancer progression.