Abcam, ab250761, Anti-MAD1L1/MAD1 antibody [EPR14676-23] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MAD1L1/MAD1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14676-23,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250761 is the carrier-free version of
ab184560
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MAD1L1 also known as MAD1 is a mitotic arrest deficient-like protein 1. It plays a critical role in cell cycle checkpoint control. MAD1L1 acts as an important component in the spindle assembly checkpoint (SAC) ensuring proper chromosome alignment and segregation during mitosis. This protein weighs roughly 85 kDa and locates primarily in the nucleus. It is expressed in various tissues with higher prevalence in rapidly dividing cells.
Biological function summary
MAD1L1 contributes to cellular homeostasis by preventing aneuploidy through its function in SAC. It participates in a larger protein complex with MAD2 another core component of SAC. Together MAD1L1 and MAD2 inhibit the anaphase-promoting complex/cyclosome (APC/C) delaying the onset of anaphase until all chromosomes align at the metaphase plate ensuring correct chromosome distribution to daughter cells.
Pathways
MAD1L1 influences the cell cycle and mitotic pathways. It interacts with other proteins like BUBR1 and CDC20 to maintain proper checkpoint function. These interactions place MAD1L1 at an important point in the pathway regulating cell division acting as both a sensor and a signal transducer to maintain genomic stability and prevent premature separation of sister chromatids.
MAD1L1 dysfunction can become associated with cancer and chromosomal instability disorders. Its aberrant expression or mutation can disrupt the accurate segregation of chromosomes leading to aneuploidy a hallmark in many types of cancer. MAD1L1 connects to key proteins like p53 and aurora kinases which also participate in maintaining genomic integrity and are often implicated in oncogenesis when mutated or dysregulated.