Abcam, ab250794, Anti-GCAP2 antibody [EPR14571] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GCAP2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14571,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250794 is the carrier-free version of
ab184958
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Guanylyl cyclase-activating protein 2 (GCAP2) also known as GUCA1B is a calcium-binding protein with a molecular weight of approximately 24 kDa. GCAP2 plays an important role in the visual system's phototransduction process. It is primarily expressed in the retina especially in photoreceptor cells. GCAP2 is part of a family of neuronal calcium sensor proteins that adjust their activities based on calcium concentration changes. These adjustments help regulate the sensitivity of photoreceptors to light.
Biological function summary
This protein modulates the activity of retinal guanylyl cyclases. In photoreceptors it forms complexes with these enzymes to regulate the synthesis of cyclic GMP in response to light. The availability of cyclic GMP is essential for the recovery phase of phototransduction which allows visual cells to respond to light changes efficiently. GCAP2 switching between active and inactive states adjusts the production of cyclic GMP depending on calcium levels inside the cells.
Pathways
GCAP2 features in the phototransduction cascade an essential pathway for converting light into visual signals. This cascade involves several proteins including rhodopsin transducin and phosphodiesterase. GCAP2’s role focuses on regulating guanylyl cyclases allowing the restoration of cyclic GMP levels which is important for returning ion channels to their open state after photon absorption. It is tightly connected to GCAP1 another guanylyl cyclase-activating protein with similar functions but they operate under slightly different calcium concentrations.
GCAP2 mutations have been linked to certain forms of cone and cone-rod dystrophies retinal degenerative disorders that affect vision. Changes in GCAP2 function influence cyclic GMP levels disrupting normal photoreceptor activity and survival. Mutations can interact with the rhodopsin promoter influencing disease progression and severity. Understanding GCAP2’s role provides potential targets for treating these retinal disorders and improving photoreceptor health.