Abcam, ab250798, Anti-GNE antibody [EPR15058] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GNE antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15058,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250798 is the carrier-free version of
ab184963
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GNE also called glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase is a bifunctional enzyme with a mass of approximately 82 kDa. This enzyme catalyzes the first two steps in sialic acid biosynthesis. GNE expression occurs in many tissues highest in liver and kidney and also in heart muscle and brain. Its bifunctional nature arises from two domain activities that facilitate the essential conversion of UDP-N-acetylglucosamine to N-acetylneuraminic acid a precursor for sialic acids.
Biological function summary
Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase acts as an important enzyme in sialic acid production acting alone and not part of a larger enzymatic complex. Sialic acids are terminal sugars on glycoproteins and glycolipids influencing cell-cell interaction signaling and stability. The enzyme's function affects cell surface structures impacting processes like cell migration and immune response.
Pathways
The enzyme GNE plays an important role in the sialylation pathway affecting various biological systems. This pathway involves the modification of glycoproteins and glycolipids critical for maintaining cellular communication. UDP-N-acetylglucosamine and N-acetylneuraminic acid are intermediates in pathways that also involve proteins like CMP-sialic acid transporter which mediates the transport of sialic acids into the Golgi apparatus for post-translational modification processes.
Defects in GNE are linked to conditions such as hereditary inclusion body myopathy (HIBM) an autosomal recessive muscle-wasting disorder. Another associated condition is sialuria a rare metabolic disorder tied to excessive levels of sialic acid. In HIBM mutations affect the enzyme’s kinase activity leading to reduced sialylation of glycoproteins. Research shows a relationship between GNE and proteins like sialin whose function as a transporter can impact the pathological manifestation of these disorders.