Abcam, ab250823, Anti-RRP7A antibody [EPR14412] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RRP7A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14412,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250823 is the carrier-free version of
ab185225
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RRP7A also known as Ribosomal RNA Processing 7 Homolog A is a protein involved in the assembly of ribosomal subunits. It has a molecular mass of approximately 33 kDa. RRP7A localizes mainly in the nucleolus where ribosomal RNA (rRNA) synthesis and ribosome assembly occur. It exists in various organisms indicating its conserved function across species. The protein is encoded by the RRP7A gene and its expression is observed in many tissues reflecting its essential function in the basic cellular process of ribosome production.
Biological function summary
RRP7A plays an important role in ribosome biogenesis an essential process for the synthesis of proteins. It is part of the small-subunit (SSU) processome complex which is involved in the maturation of 18S rRNA a critical component of the small ribosomal subunit. The SSU processome is essential for early steps of 40S ribosomal subunit assembly. RRP7A acts in association with other proteins within this complex to facilitate the accurate processing of pre-rRNA into functional rRNA molecules necessary for proper ribosomal function.
Pathways
Ribosomal RNA processing represents a critical aspect of translation and protein synthesis. RRP7A fits into the ribosome biogenesis pathway ensuring the correct formation and function of ribosomes. This pathway involves several other proteins including FBL (Fibrillarin) and DDX5 which work alongside RRP7A within the SSU processome. Together these components contribute to the intricate steps of rRNA processing from transcription to the assembly and transport of ribosomal subunits. This makes RRP7A integral to maintaining cellular protein synthesis capacity.
Impaired function of RRP7A can be related to ribosomopathies and certain cancer types due to disruptions in ribosome production. In some cancers altered ribosome biogenesis results in increased cellular proliferation. RRP7A disruptions link to proteins such as MYC a well-known oncogene that can promote ribosome biogenesis when overexpressed. Understanding RRP7A's role in these conditions can inform studies exploring the therapeutic potential of targeting ribosome biogenesis pathways in disease treatment.