Abcam, ab250858, Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SAE2 / UBA2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14880,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250858 is the carrier-free version of
ab185955
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SAE2 also known as UBA2 is a protein involved mechanically in the SUMO (Small Ubiquitin-like Modifier) conjugation pathway. It functions as an E1 activating enzyme collaborating closely with its partner SAE1 in the initiation of SUMOylation. SAE2 has a known molecular mass of approximately 72 kDa. It is widely expressed in various tissues with high expressions noted in the nucleus. This expression pattern suggests it plays a role in maintaining cellular processes under a variety of conditions.
Biological function summary
This protein plays an important role in the post-translational modification process of SUMOylation which is critical for regulating protein function. SAE2 is part of the E1 activating enzyme complex working specifically with SAE1 to activate and transfer SUMO to target proteins. It contributes to cellular processes such as nuclear-cytosolic transport chromosome segregation and DNA repair. This indicates its functions are central to maintaining genomic stability and cellular homeostasis.
Pathways
SAE2 is integral in the SUMOylation pathway which interacts with critical cellular pathways like the DNA damage response and cell cycle regulation pathways. It has important interactions with proteins such as UBE2I (also known as Ubc9) the only E2 enzyme in the SUMOylation cascade and PIAS family proteins which function as E3 ligases. These connections allow SAE2 to influence various pathways essential for cell survival and proliferation.
SAE2 influences pathological conditions such as cancer and neurodegenerative diseases. Its role in SUMOylation connects it to the regulation of oncogenic pathways potentially linking it with proteins like p53 a well-known tumor suppressor. In neurodegenerative diseases alterations in SUMOylation pathways with SAE2 as a participant might affect proteins involved in neuron function and survival contributing to disease progression. Understanding SAE2's interactions in these diseases might provide insights into therapeutic strategies.