Abcam, ab250909, Anti-MED7 antibody [EPR15410] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MED7 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15410,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250909 is the carrier-free version of
ab187146
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MED7 also known as Mediator Complex Subunit 7 is a protein component of core Mediator complex essential for transcription regulation by RNA polymerase II. MED7 weighs approximately 25 kDa and exists in nucleus. It is part of a large multi-protein assembly. This subunit participates specifically by aiding in the mediator's interactions with gene-specific regulatory proteins and general transcription factors essential for transcription initiation.
Biological function summary
These components coordinate as part of Mediator complex which regulates transcription by acting as bridge between DNA-bound transcriptional regulators and RNA polymerase II. MED7 contributes to the structural integrity of Mediator complex affecting transcriptional activation processes necessary for gene expression. The protein's roles link it directly to cell growth differentiation and response to various stimuli indicating its importance in diverse cellular processes.
Pathways
MED7 integrates itself into transcription regulation pathways especially interacting with other subunits like MED1 and MED14. The Mediator complex functions in the pathway responsible for transferring information from transcription factors to RNA polymerase II. This involves interacting with multiple pathways including Notch signaling and Wnt signaling serving as coordinator between transcriptional regulators and transcription machinery.
MED7 has associations with cancer and neurodevelopmental disorders. Aberrations in Mediator complex components including MED7 can impact transcriptional dysregulation linked to oncogenesis. Mutations in this subunit along with changes in associated proteins such as MED12 are observed in various cancer types. Additionally disturbances in Mediator complex function potentially involving MED7 contribute to neurodevelopmental conditions due to misregulation of gene expression essential for neural development.