Abcam, ab250984, Anti-UFC1 antibody [EPR15014-102] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal UFC1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15014-102,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250984 is the carrier-free version of
ab189252
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UFC1 also known as ubiquitin-fold modifier-conjugating enzyme 1 or UFM1-conjugating enzyme 1 functions mechanically by facilitating the conjugation of ubiquitin-fold modifier 1 (UFM1) to target substrates. It exhibits a molecular mass of approximately 20.8 kDa. UFC1 is expressed in a variety of tissues including liver kidney and heart indicating its widespread role in cellular processes. As an essential component in the UFM1 system UFC1 works closely with other proteins to regulate protein modification through UFMylation.
Biological function summary
UFC1 is involved in the process of UFMylation which modifies proteins through the attachment of UFM1 much like ubiquitination influences protein degradation and signaling. UFC1 forms part of a complex that also includes UBA5 and UFL1 the E1 activating enzyme and E3 ligase respectively. This UFMylation system is necessary for endoplasmic reticulum-associated degradation (ERAD) contributing to the maintenance of protein homeostasis within cells. The disruption of this modification process can impact cellular stress response and protein quality control mechanisms.
Pathways
UFC1 plays a critical role in the ER stress response and protein quality control pathways. Within these pathways UFC1 interacts with proteins such as UFM1 and UFL1 to mediate the UFMylation of target proteins during cellular stress. Additionally UFC1 has been linked to pathways regulating autophagy where it indirectly influences the degradation of defective proteins. This interaction ultimately affects the cellular response to misfolded proteins and is pivotal for maintaining cellular equilibrium.
UFC1 has implications in conditions such as cancer and neurological disorders. Abnormalities in UFMylation can contribute to the dysregulation of protein homeostasis which is frequently observed in cancerous cells. In the context of neurological disorders the dysfunctional UFM1 system involving UFC1 can result in impaired neuronal homeostasis. Additionally UFC1's relationship with proteins like UFM1 and UFL1 highlights its importance in the pathological processes of these diseases positioning it as a potential target for therapeutic intervention.