Abcam, ab250994, Anti-AFG2 antibody [EPR14038] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal AFG2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14038,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250994 is the carrier-free version of
ab189519
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AFG2 also known as ATP-dependent metalloprotease FtsH2 is an essential protein involved in protein quality control within mitochondria. This protein acts mechanically as a protease enabling the degradation of misfolded or damaged proteins preventing accumulation within the mitochondrial matrix. AFG2 has a molecular mass of approximately 80 kDa. It is expressed across various tissues predominantly in those with high-energy demands such as cardiac and skeletal muscles.
Biological function summary
FtsH2 forms part of the m-AAA protease complex. This complex consists of other subunits that work together to maintain mitochondrial function. AFG2 contributes to mitochondrial protein homeostasis by degrading unassembled or faulty proteins. It also plays a significant role in the processing of mitochondrial ribosomal proteins ensuring efficient ribosome function and mitochondrial protein synthesis.
Pathways
AFG2 contributes significantly to mitochondrial biogenesis and the oxidative phosphorylation system. It interacts with other proteins such as paraplegin to regulate mitochondrial function and network maintenance. AFG2 ensures proteostasis within the mitochondria directly impacting cellular energy production. Its involvement in pathways ensures the balance of mitochondrial dynamics influencing cellular metabolism and energy distribution.
Alterations in AFG2 activity often associate with neurodegenerative diseases such as hereditary spastic paraplegia and spinocerebellar ataxia. Mutations or dysregulation of AFG2 can disrupt mitochondrial proteostasis and function leading to these conditions. Additionally the protein is linked to paraplegin whose mutations may result in similar mitochondrial dysfunction emphasizing the interconnectedness of mitochondrial proteases in maintaining neurological health.