Abcam, ab251033, Anti-ATP6V1F antibody [EPR15053] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ATP6V1F antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15053,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251033 is the carrier-free version of
ab190789
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP6V1F also known as Vacuolar ATPase subunit F is a component of the V-type ATPase (V-ATPase) complex. This protein has a molecular mass of approximately 13 kDa. ATP6V1F is expressed in various tissues with higher levels observed in kidney heart and skeletal muscle tissues. It plays a mechanical role in proton transport across the vacuolar membrane important for acidifying cellular organelles such as endosomes lysosomes and the Golgi apparatus.
Biological function summary
ATP6V1F plays a critical part in cellular processes such as protein degradation ion homeostasis and membrane trafficking. ATP6V1F is a part of the V-ATPase complex which is essential for maintaining the acidification of intracellular compartments. The proton pump activity of V-ATPase involves ATP hydrolysis where ATP6V1F contributes to its regulation and structural stability. It helps in cellular events that require compartmental pH regulation impacting multiple physiological roles.
Pathways
ATP6V1F interacts with processes like endocytosis and lysosome-mediated degradation. The V-ATPase complex which includes ATP6V1F functions in conjunction with proteins such as Rab5 and clathrin during endocytic pathways and is implicated in mTOR signaling. These interactions underline its involvement in both nutrient sensing and cellular metabolism indicating its significance in cellular nutrient dynamics and homeostasis.
ATP6V1F connects to conditions like neurodegenerative diseases and osteopetrosis. Variations in this protein or its dysfunction can lead to improper acidification of lysosomes contributing to neurological disorders through aggregation of misfolded proteins. Additionally its role in osteoclast-mediated bone resorption relates ATP6V1F to osteopetrosis where excessive bone density occurs due to failed bone resorption. The protein complex V-ATPase and its subunits like ATP6V0D1 and ATP6V1B2 often closely correlate with these conditions illustrating a network of related proteins influencing similar pathways and disease outcomes.