Abcam, ab251070, Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NDUFAF4 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15931,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251070 is the carrier-free version of
ab191414
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDUFAF4 also known as Complex I Assembly Factor 2 plays an integral role in the assembly of NADH:ubiquinone oxidoreductase complex I within mitochondria. It has a mass of approximately 20 kilodaltons. This protein is widely expressed in various tissues with heightened levels found in organs with high energy demands such as the brain and heart. Functionally NDUFAF4 mediates the proper formation and stabilization of the mitochondrial complex I by contributing to the assembly of its subunits.
Biological function summary
NDUFAF4 participates in the mitochondrial electron transport chain as part of the complex I assembly. It acts as an assembly factor for complex I the first component of the chain which is important for initiating electron transport from NADH to the rest of the chain. This complex contains multiple subunits that require precise assembly and NDUFAF4 ensures the correct incorporation of these subunits. Its role is essential for maintaining efficient mitochondrial respiration thereby supporting ATP production.
Pathways
The NDUFAF4 protein ensures proper electron transport chain function by facilitating complex I assembly. It interacts within mitochondrial pathways involving oxidative phosphorylation and reactive oxygen species (ROS) regulation. It associates with other proteins such as NDUFS3 and NDUFS4 as part of the complex I formation process. Progressive assembly of complex I facilitated by NDUFAF4 is an important step in maintaining cellular energy metabolism.
Improper function or expression of NDUFAF4 leads to mitochondrial disorders including Leigh syndrome and other metabolic encephalopathies. Mutations in NDUFAF4 disrupt complex I assembly resulting in impaired mitochondrial respiration and energy production. This protein also relates to Parkinson’s disease where its involvement in complex I assembly connects it to proteins like Parkin which are associated with mitochondrial dysfunction in dopaminergic neurons.