Abcam, ab251081, Anti-Arfaptin-1 antibody [EPR16360] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Arfaptin-1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16360,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251081 is the carrier-free version of
ab191430
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Arfaptin-1 also known as ARFIP1 acts mechanically by modulating membrane dynamics and cell signaling. This protein with a mass of approximately 36 kDa associates with membranes through a BAR domain which induces curvature. It interacts with small GTPases including ARF and Rac1 to facilitate various intracellular trafficking processes. Arfaptin-1 expression occurs in multiple tissue types suggesting its wide role in cellular functions.
Biological function summary
Arfaptin-1 plays a part in intracellular transport and membrane receptor regulation. It is also recognized as a component of larger protein complexes contributing to the reorganization of actin cytoskeleton. Through its interactions with GTPases Arfaptin-1 influences endocytosis and exocytosis affecting cell surface receptor turnover. This adaptability allows it to play important roles in cellular communication and response to environmental stimuli.
Pathways
Arfaptin-1 is integral in cellular trafficking and signal transduction pathways. It is involved in the ARF small GTPase pathway influencing vesicle formation and trafficking. Arfaptin-1 also interacts with other proteins like Rac1 in the regulation of actin dynamics impacting the actin cytoskeleton remodeling pathway. These interactions highlight its central position in coordinating cellular architecture and material transport.
Arfaptin-1 involvement links to conditions like cancer and neurological disorders. Altered expression of Arfaptin-1 has been observed in cancer cells where it affects cell migration and invasion linking with other proteins such as Rac1. In neurological disorders abnormal Arfaptin-1 function disrupts synaptic vesicle trafficking potentially interacting with proteins involved in neurodegenerative diseases. These associations emphasize its potential as a target in therapeutic strategies.