Abcam, ab251095, Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal REDD-1/DDIT4 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18716,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251095 is the carrier-free version of
ab191871
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
REDD-1 also known as DDIT4 (DNA Damage Inducible Transcript 4) is a protein involved in cellular response to stress particularly hypoxic conditions. It has a molecular weight of approximately 35 kDa. This protein is expressed in various tissues including the brain liver and muscle indicating its importance in different physiological environments. REDD-1 acts as a negative regulator of the mechanistic target of rapamycin (mTOR) signaling pathway which plays an important role in cell growth and survival.
Biological function summary
The role of REDD-1 involves its participation in cellular adaptation to stress by inhibiting mTORC1 a complex within the mTOR pathway. Through this mechanism it helps cells to survive in limiting conditions by adjusting metabolic and growth signals. REDD-1 is not part of a larger protein complex but interacts closely with components of the mTOR pathway. Its regulation ensures proper cellular function under stress making it an important point for controlling cell behavior.
Pathways
REDD-1 serves as a significant regulator in the mTOR signaling and cellular stress response pathways. REDD-1 inhibits mTORC1 activity facilitating the cellular adaptation under nutrient starvation and hypoxic conditions. This inhibition affects the phosphorylation of downstream targets like S6K1 and 4EBP1 which are important for protein synthesis. Moreover REDD-1's relationship with the AMP-activated protein kinase (AMPK) adds another layer of regulation in energy balance and metabolic control.
REDD-1's dysregulation links to cancer and neurodegenerative diseases. In cancer altered REDD-1 expression affects mTORC1 signaling which can lead to unregulated cell growth and proliferation. The protein is connected to hypoxia-inducible factor (HIF) which contributes to tumor growth in hypoxic environments. In neurodegenerative diseases improper stress responses involving REDD-1 may exacerbate neuronal damage. Understanding REDD-1's interactions with mTORC1 and HIF could offer insights into potential therapeutic targets for these conditions.