Abcam, ab251103, Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ARHGAP25 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13233-60,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251103 is the carrier-free version of
ab192020
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARHGAP25 also known as Rho GTPase-activating protein 25 functions mechanically as a GTPase activator for the Rho-family of small GTPases. This protein plays a role in switching these GTPases from an active state to an inactive state by stimulating the intrinsic GTPase activity. ARHGAP25 has a mass of approximately 61 kDa. It is expressed mainly in hematopoietic cells with specific expression in leukocytes. Its activity is important in cellular processes involving actin cytoskeletal reorganization.
Biological function summary
ARHGAP25 modulates the actin cytoskeleton impacting cell migration phagocytosis and other hematopoietic cell functions. It acts as a part of regulatory complexes that control cytoskeletal dynamics and cellular morphology. By regulating Rho family members like Rac1 and Cdc42 ARHGAP25 influences actin polymerization and cellular movement particularly in immune cells. This protein contributes to maintaining the integrity of immune responses.
Pathways
ARHGAP25 plays a role in the Rho GTPase signaling pathway and interacts with pathways that control cell division and migration. Within these networks ARHGAP25 interacts closely with proteins such as Rac1 and Cdc42 which are widely involved in actin dynamics and cell motility. This interaction impacts pathways that govern the reorganization of actin filaments important for leukocyte function and migration.
ARHGAP25 is involved in conditions like chronic inflammatory diseases and immune system dysregulation. Its dysregulated expression or function can lead to altered immune cell behavior and contribute to chronic inflammation. The protein's relationship with Rac1 and Cdc42 is significant as these proteins also associate with pathways implicated in immune dysfunction and inflammation. Understanding ARHGAP25's role provides insight into targeting therapies for disorders characterized by abnormal immune responses.