Abcam, ab251105, Anti-TSPEAR antibody [EPR15505] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TSPEAR antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15505,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251105 is the carrier-free version of
ab192023
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TSPEAR also known as Thrombospondin Type S Serine Protease and ARM Repeat Containing is a protein with a molecular mass of approximately 92 kDa. It functions mechanically as a protein involved in cell adhesion and communication processes. Expression of TSPEAR occurs predominantly in epithelial tissues including the skin and the gastrointestinal tract. It contains a series of ARM repeats which contribute to its functional role within the cellular structure.
Biological function summary
It participates in essential signaling pathways that regulate cell proliferation and differentiation. TSPEAR often associates with other proteins forming a complex that mediates interactions between cells and the surrounding extracellular matrix. This function is important for maintaining the stability of tissue architecture and facilitating tissue development and repair processes.
Pathways
TSPEAR plays a role in processes like the Notch signaling and Wnt signaling pathways. In the Notch signaling pathway it interacts with proteins involved in cell fate determinations assisting in the regulation of cell differentiation. Within the Wnt pathway TSPEAR associates with other proteins to influence cell growth and development. Its interaction with these pathways implies a cooperative role with proteins such as Notch receptors and beta-catenin highlighting its involvement in critical cellular communication networks.
TSPEAR has been linked to conditions like Alagille syndrome and certain skin disorders. In Alagille syndrome it interacts with proteins such as JAG1 and NOTCH2 which are important for the proper signaling required in fetal development. Moreover its expression levels can alter in various dermatological conditions connecting TSPEAR to proteins involved in skin structure and repair. Understanding its role in these disorders may provide insights into therapeutic targets and interventions.