Abcam, ab251107, Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HCN3 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13494(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251107 is the carrier-free version of
ab192025
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Hyperpolarization-activated Cyclic Nucleotide-gated channel 3 (HCN3) also known as potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 is a protein that forms ion channels. These channels facilitate the flow of sodium and potassium ions across the cell membrane which is essential for maintaining the electrical activity in cells. HCN3 has a molecular mass of approximately 97 kDa and is expressed in various tissues including the brain and heart. Its role in creating the 'funny current' makes it important for electrical rhythms in these tissues.
Biological function summary
HCN3 channels contribute to pacemaker activities in neurons and cardiac cells regulating heartbeat and neural firing. HCN3 does not operate in isolation; it can be part of larger channel complexes influencing the excitability of cells. These channels act by responding to membrane hyperpolarization opening to allow ion flow that modulates cell activity and therefore impacts processes like synaptic transmission and cardio-excitatory responses.
Pathways
HCN3 channels integrate into signal transduction pathways involving cyclic nucleotides specifically the cAMP pathway. They interact with other hyperpolarization-activated cyclic nucleotide-gated channels like HCN1 and HCN2 which help refine electrical signaling. HCN3's involvement in the regulation of neural excitability links it to pathways governing neurotransmitter release and heart rate modulation.
HCN3 potentially relates to epilepsy and cardiac arrhythmias. Aberrant HCN3 channel function can disrupt electrical signaling contributing to pathogenesis in these conditions. Interactions with other ion channels such as HCN2 may amplify disturbances in neural firing and cardiac pacing. Understanding HCN3’s functional mechanics offers insight into its role in disease manifestation and could guide therapeutic targeting.