Abcam, ab251133, Anti-IPP antibody [EPR15575] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal IPP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15575,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251133 is the carrier-free version of
ab192266
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The molecule isopentenyl pyrophosphate (IPP) sometimes referred to as isopentenyl diphosphate serves as an important intermediate in the biosynthesis of terpenes and isoprenoids. IPP a small molecule with a molecular mass of 246.1 g/mol participates in various enzymatic reactions within the mevalonate pathway. This pathway occurs in the cytosol of eukaryotic cells and the peroxisomes in plants. In terms of its expression while IPP is not a protein or gene with specific expression levels the enzymes related to its synthesis and utilization show varying activity across different tissues.
Biological function summary
IPP functions as a precursor for various essential isoprenoid compounds encompassing sterols dolichols and ubiquinones. It acts within a network of enzymes converting basic building blocks into complex molecules that maintain cell membranes and facilitate electron transport. These reactions often involve enzyme complexes like synthases that regulate the flow of intermediates. Moreover IPP plays a substantial role in the synthesis of other molecules like cholesterol and steroid hormones reflecting its broad biological influence.
Pathways
IPP engages prominently in the mevalonate and the methylerythritol phosphate (MEP) pathways. The mevalonate pathway produces isoprenoids which are vital for cell growth and development. Within these pathways IPP can interconvert with dimethylallyl pyrophosphate (DMAPP) another critical molecule. Enzymes like HMG-CoA reductase and isopentenyl-diphosphate isomerase control these reactions linking IPP to key biological pathways involved in metabolic regulation and cellular differentiation.
IPP's significance links to disorders related to cholesterol metabolism and immune system dysregulation. Defects in the mevalonate pathway associate with diseases like mevalonate kinase deficiency influencing levels of inflammatory proteins. Moreover IPP accumulation can activate immune cells through the recognition by Vγ9/Vδ2 T-cells implicating it in conditions such as autoimmunity or inflammation. This connection highlights the consequence of IPP in understanding metabolic and immune-related disorders.