Abcam, ab251152, Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Pellino 1 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19282,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251152 is the carrier-free version of
ab192833
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pellino 1 and Pellino 2 are E3 ubiquitin ligases and known as PELI1 and PELI2 respectively. PELI1 has a mass of approximately 42 kDa while PELI2 has a similar size around 40 kDa. These proteins are expressed in various tissues including immune cells where they play important roles in cellular signaling processes. Both Pellino proteins contain RING-like domains which facilitate their role in protein ubiquitination a process tagging proteins for degradation or modulating their function.
Biological function summary
Pellino proteins are involved in immune response modulation. They influence signaling pathways such as Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) pathways. Pellino proteins participate in forming complexes with other signaling proteins assisting in the propagation of inflammatory responses. Their ability to link with components like IRAK1 and TRAF6 helps regulate these immune signaling cascades critical for innate immunity.
Pathways
Pellino proteins are involved in the TLR and IL-1R pathways which are key to innate immune responses. These pathways often include the activation of NF-kB a major transcription factor that regulates immune and inflammatory responses. Pellino proteins interact with signaling proteins like IRAK1 leading to the further propagation of signals that culminate in the activation of NF-kB and the production of pro-inflammatory cytokines.
Pellino proteins are connected to conditions like rheumatoid arthritis and systemic lupus erythematosus. In rheumatoid arthritis aberrant signaling pathways involving Pellino proteins contribute to chronic inflammation and tissue damage. Through their interaction with IRAK1 Pellino proteins can affect downstream signaling that exacerbates auto-inflammatory responses. Their dysregulation can therefore link directly to mechanisms underpinning these autoimmune diseases.