Abcam, ab251154, Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal C14orf169 / NO66 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18656,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251154 is the carrier-free version of
ab192861
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C14orf169 also known as NO66 is a nuclear protein implicated in chromatin regulation and protein synthesis. It has an estimated molecular mass of approximately 85 kDa. This protein shows widespread expression in various tissues including high levels in the brain and liver. C14orf169 possesses JmjC domain which provides it with demethylase activity influencing histone modifications that are critical for chromatin structure and gene expression regulation.
Biological function summary
C14orf169 functions as a part of nuclear complexes involved in transcriptional regulation. It interacts with other chromatin-associated factors ensuring proper histone modification which is necessary for modulating gene expression. This protein's role in regulating chromatin's epigenetic state connects it to important cellular processes such as cell cycle progression and response to stress.
Pathways
C14orf169 integrates within histone modification and transcriptional regulation pathways. Its demethylase activity influences the H3K4me3 and H3K36me3 marks important for transcriptional activation or repression. C14orf169 shows interactions with other proteins such as histone demethylases and methyltransferases that cooperate in modifying chromatin structure affecting gene transcription patterns.
C14orf169's dysregulation associates with cancer types including prostate cancer. Changes in its expression may lead to altered chromatin states affecting oncogene and tumor suppressor gene regulation. Additionally C14orf169 connects with ubiquitin-proteasome pathways implicating it in neurodegenerative disorders where protein degradation becomes impaired specifically in conditions like Alzheimer's disease.