Abcam, ab251173, Anti-UCP3+UCP1 antibody [EPR19006] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal UCP1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19006,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251173 is the carrier-free version of
ab193470
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Uncoupling proteins UCP3 and UCP1 are members of the mitochondrial anion carrier protein family. These proteins play significant roles in mitochondrial proton leak impacting the production of ATP and regulation of energy balance. UCP1 also known as thermogenin has a molecular mass of approximately 32 kDa and is primarily expressed in brown adipose tissue. In contrast UCP3 is found mostly in skeletal muscle and is slightly larger around 33 kDa. Both proteins facilitate the uncoupling process in mitochondria dissipating proton gradient energy as heat instead of ATP synthesis.
Biological function summary
UCP3 and UCP1 are involved in thermoregulation and energy metabolism. These proteins do not operate as part of a larger protein complex but contribute to distinct cellular processes. UCP1 generates heat in brown adipose tissue enabling thermogenesis a process important for maintaining body temperature in cold environments. UCP3 plays a role in regulating fatty acid metabolism and mitigating the effects of reactive oxygen species within muscle cells. This protein may protect mitochondria from damage due to oxidative stress.
Pathways
The roles UCP3 and UCP1 play intersect significantly with the oxidative phosphorylation and lipid metabolism pathways. UCP1 links closely to the thermogenesis pathway where it aids in converting stored energy in brown fat into heat alongside signaling proteins like PGC-1α. UCP3 integrates into pathways that involve the oxidation of fatty acids working with proteins such as peroxisome proliferator-activated receptors (PPARs) to regulate energy combustion in muscle tissues.
UCP3 and UCP1 have connections to obesity and type 2 diabetes. Low UCP1 activity can result in reduced thermogenesis and increased susceptibility to obesity with leptin and insulin involved in this dysfunctional process. UCP3 variations have associations with impaired fatty acid oxidation contributing to lipid accumulation in muscles and insulin resistance a prominent issue in type 2 diabetes. Understanding the modulation of these proteins offers avenues for therapeutic intervention in metabolic disorders.