Abcam, ab251176, Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ATP1B1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12195,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251176 is the carrier-free version of
ab193669
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP1B1 also known as the beta-1 subunit of Na+/K+ ATPase plays an essential role in ion transport across the plasma membrane. The protein works as part of the sodium-potassium pump which helps maintain the cellular electrochemical gradient. The molecular mass of ATP1B1 is approximately 35 kDa. This target is expressed in various tissues including the heart kidney and brain where it supports the critical functions of excitable tissues and epithelial cell polarization.
Biological function summary
ATP1B1 contributes to establishing and maintaining cellular homeostasis as part of the Na+/K+ ATPase complex. This complex regulates the balance of sodium and potassium ions within cells important for nerve impulse transmission and muscle contraction. ATP1B1 interacts with the alpha subunit to modulate the enzyme’s activity and transport kinetics highlighting its role in cell volume regulation and epithelial layer development.
Pathways
ATP1B1 is actively involved in the ion transport pathway and cellular signaling pathways such as WNT signaling. It works closely with proteins such as ATP1A1 and ATP1A2 which are different alpha subunits of the Na+/K+ ATPase. Through these pathways ATP1B1 affects cellular processes like membrane potential maintenance and intercellular communication essential for proper cellular function and organismal health.
ATP1B1 shows a link to hypertension and polycystic ovary syndrome (PCOS). Mutations or dysfunctional expression of ATP1B1 can disrupt ion gradients contributing to these conditions. The protein connects with regulatory proteins including G-protein signaling regulators which can alter cellular responses in disease contexts. Understanding ATP1B1's involvement in these disorders helps in exploring therapeutic targets and improving patient outcomes.