Abcam, ab251201, Anti-TAF5 antibody [EPR15433] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TAF5 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15433,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251201 is the carrier-free version of
ab195236
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TAF5 also known as TAFII100 is a component of the transcription factor IID complex and is vital in the process of assembling the pre-initiation complex for gene transcription. This protein has a molecular mass of approximately 100 kDa. It is prominently expressed in a variety of tissues such as liver and heart indicating its broad functional significance in various cellular environments. TAF5 through its structural role helps stabilize the TFIID complex facilitating the binding of transcription machinery to DNA promoters.
Biological function summary
TAF5 forms part of the TFIID complex a cornerstone for gene transcription initiation. TFIID is one of the basal transcription factors essential for RNA polymerase II to carry out transcription of mRNA. The proper assembly and functioning of TFIID rely heavily on TAF5 as it interacts with other TAFs and the TBP (TATA-box Binding Protein) enabling accurate transcriptional regulation. This process impacts the expression of a large number of genes highlighting TAF5's broad biological utility and influence.
Pathways
TAF5 plays a major role in the transcription initiation pathway and is an active participant in the RNA polymerase II transcription machinery. It associates closely with TAF6 and TAF9 within the TFIID complex facilitating the recruitment of other proteins to initiate transcription. Through these interactions TAF5 impacts the cell cycle control and apoptosis pathways modulating cellular responses to developmental cues and environmental stresses. These pathways illustrate the importance of TAF5 in maintaining cellular homeostasis and responding to physiological signals.
TAF5 has associations with cancer and certain neurodegenerative diseases. Aberrant expression or mutations in the TAF5 gene may disrupt the transcriptional machinery leading to uncontrolled cellular proliferation or neuronal cell death. For example in cancer TAF5's interaction with TIP60 a histone acetyltransferase modifies chromatin structure influencing tumorigenic processes. Meanwhile in neurodegenerative disorders impaired TAF5 functions might alter gene expression patterns important for neuronal survival linking TAF5 to the disease pathology and progression.