Abcam, ab251245, Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Ribophorin I antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17044(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251245 is the carrier-free version of
ab197888
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ribophorin I also known as RPN1 is a glycoprotein with a mass of approximately 68 kDa. It is located in the rough endoplasmic reticulum (ER) and is part of the oligosaccharyltransferase complex which is involved in the N-linked glycosylation of nascent polypeptides. This protein facilitates the unanimous transfer of oligosaccharides to asparagine residues in polypeptide chains an important step in protein maturation. Ribophorin I shows expression in various tissues but is especially prominent in the liver and pancreas where high levels of protein synthesis occur.
Biological function summary
Ribophorin I plays an essential role in the process of protein folding and quality control within the ER. It is part of the oligosaccharyltransferase complex along with other key proteins like STT3A/B and Ribophorin II. The complex ensures that nascent proteins receive the proper glycosylation necessary for proper folding and stability. This process helps maintain a balance between protein production and degradation ensuring that only properly folded proteins proceed to their intended cellular locations.
Pathways
Ribophorin I functions within the N-linked glycosylation pathway critical for protein maturation and function. Also it takes part in the unfolded protein response pathway which reacts to stress conditions that disrupt the folding processes in the ER. This pathway involves other proteins such as BiP/GRP78 and ATF6 which work together to restore normal protein folding conditions. Ribophorin I's efficient function in these pathways is key to sustaining cellular operations maintaining protein homeostasis.
Ribophorin I is implicated in cancer and neurodegenerative diseases. Its malfunction can disrupt protein glycosylation and folding contributing to the progression of cancer by allowing aberrant glycoproteins on the cell surface. In neurodegenerative disorders like Alzheimer's disease impaired N-linked glycosylation affects protein folding leading to aggregation of misfolded proteins. Ribophorin I interacts indirectly with proteins such as amyloid precursor protein (APP) in Alzheimer's influencing the disease pathology through related glycosylation processes.