Abcam, ab251299, Anti-RDH5 antibody [EPR15191] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RDH5 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15191,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251299 is the carrier-free version of
ab200197
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RDH5 also known as retinol dehydrogenase 5 or 11-cis retinol dehydrogenase is an enzyme that plays an important role in the visual cycle. It has a molecular mass of approximately 35 kDa and primarily functions in the conversion of 11-cis-retinol to 11-cis-retinal which is a significant step in the biosynthesis of visual pigments. RDH5 is expressed mainly in the retinal pigment epithelium of the eye where it contributes to maintaining proper vision.
Biological function summary
11-cis retinol dehydrogenase facilitates the regeneration of 11-cis-retinal from 11-cis-retinol a critical process for photoreceptor cell function and visual cycle continuity. RDH5 operates as part of a complex of enzymes involved in the retinoid cycle which is essential for maintaining a continuous supply of 11-cis-retinal. Without RDH5 the regeneration of 11-cis-retinal would be ineffective reducing the ability to see especially in low-light conditions.
Pathways
The retinol dehydrogenase 5 enzyme plays a major role in the visual cycle pathway. This pathway is necessary for converting retinoids into visual chromophores that allow vision. RDH5 interacts closely with other key enzymes such as RPE65 and RDH12 which also participate in retinoid transformations. The proper functioning of the visual cycle pathway depends on the actions of these enzymes to ensure the availability of 11-cis-retinal for photoreceptor function.
Defects in the RDH5 gene can lead to retinal degenerative diseases like fundus albipunctatus and retinitis pigmentosa. These conditions are characterized by night blindness and progressive vision loss. Mutations in RDH5 affect the synthesis of 11-cis-retinal disrupting the visual cycle and contributing to these diseases. RDH5 shares a pathway with RPE65 another protein implicated in retinal disorders highlighting the interconnectedness of proteins involved in the visual cycle and their impact on eye health.