Abcam, ab251302, Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RBMY1A1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:R12508(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Based on sequence analysis, this antibody recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.

Product details:
ab251302 is the carrier-free version of
ab200202
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RBMY1A1 also known as RNA-binding motif protein Y chromosome family 1 member A1 is a protein with a molecular mass of about 47 kDa. It is expressed predominantly in the testis playing an important role in male germ cell development. The protein functions mechanically by binding to RNA featuring an RNA recognition motif that facilitates its role in RNA processing. Its localization to the nucleus highlights its function in post-transcriptional regulation.
Biological function summary
RBMY1A1 contributes to spermatogenesis by participating in the regulation of RNA splicing and stability. It is part of a larger complex of proteins that associate with RNA impacting gene expression in male germ cells. The protein interacts with other splicing factors influencing the alternative splicing of pre-mRNAs essential for spermatogenic processes. This highlights RBMY1A1's specific role in sperm development and functioning.
Pathways
The involvement of RBMY1A1 is significant in the mRNA processing pathway. It connects with proteins like hnRNPGT and other spliceosomal components. RBMY1A1’s interaction in this pathway helps in the formation of messenger ribonucleoprotein complexes which are necessary for the maturation and transport of mRNA molecules. Its activity is important in maintaining the integrity of gene expression regulation in spermatogenic cells intertwining with broader pathways of male fertility.
RBMY1A1 has been implicated in cases of azoospermia a condition characterized by the absence of sperm in semen. This condition associates with its critical role in fertility where loss or malfunction correlates with impaired sperm production. Moreover RBMY1A1 has links to testicular germ cell tumors where abnormal expression and mutations have been observed. It also interacts with other proteins like DAZL which is another factor associated with reproductive health emphasizing its relevance in male reproductive disorders.