Abcam, ab251316, Anti-NCAPH2 antibody [EPR17170] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NCAPH2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17170,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251316 is the carrier-free version of
ab200659
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NCAPH2 also known as non-SMC condensin II complex subunit H2 plays an important role in chromosome condensation. It is a part of the condensin II complex and contributes to the structuring and stabilization of chromatin. NCAPH2 has a mass of approximately 99 kDa and is expressed broadly in various tissues including embryonic tissues and adult organs. Its functional role is important in processes that require chromatin reorganization.
Biological function summary
NCAPH2 functions as an important component of the condensin II complex which facilitates proper chromosome segregation during cell division. The protein through its interaction with other condensin subunits C and A oversees the maintenance of chromosome architecture. It also influences mitotic progression by ensuring the accurate separation of sister chromatids. Research shows NCAPH2's involvement in maintaining genome stability highlighting its biological significance.
Pathways
NCAPH2 participates in important cellular pathways such as mitotic chromosome condensation and DNA repair mechanisms. Within these pathways NCAPH2 interacts closely with proteins like SMC2 and SMC4 which form the core of the condensin complexes. Correct functioning of these pathways ensures the integrity of genetic material during cell division preventing aneuploidy and other chromosomal abnormalities.
NCAPH2 has been linked to various conditions including cancer and developmental disorders. Its dysregulation has associations with certain cancers due to its role in maintaining genomic stability where its aberrant expression may lead to improper cell division and tumorigenesis. Additionally connections to Kleefstra syndrome where a disruption impacts chromatin organization suggest NCAPH2's interactions with other chromatin-modifying proteins including histone deacetylases.