Abcam, ab251333, Anti-HCE antibody [EPR19384] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HCE antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19384,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251333 is the carrier-free version of
ab201046
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HCE also known as Heat Shock Cognate 70 is a stress-responsive protein with a mass of approximately 70 kDa. This protein plays a mechanical role as a molecular chaperone assisting in the proper folding of nascent polypeptides and the refolding of misfolded proteins. HCE expression occurs in various tissues but notably it is abundant in the cytoplasm of cells exposed to stressful conditions like heat shock. The protein assists in maintaining cellular protein homeostasis particularly during periods of increased environmental stress.
Biological function summary
HCE contributes to multiple cellular processes critical for survival and adaptation. It is an important component of the chaperone complex and interacts with co-chaperones to facilitate protein folding and prevent aggregation. HCE enhances cellular resilience by stabilizing proteins and protecting against cellular stress-induced damage. Additionally it supports proteostasis by targeting misfolded proteins for degradation via the ubiquitin-proteasome system.
Pathways
HCE is involved in essential cellular mechanisms beyond protein folding including the unfolded protein response (UPR) and heat shock response (HSR). In these pathways HCE collaborates with proteins like HSP40 and HSP90 to restore normal protein conformation and function. The heat shock response pathway is a protective cellular mechanism that activates a cascade to manage stress-related protein damage and ensure cell survival during acute stress.
HCE has significant connections to neurodegenerative diseases such as Alzheimer’s and Parkinson’s. Research indicates that aberrant HCE function may contribute to protein aggregation a hallmark of these conditions. In Alzheimer’s disease HCE interacts with proteins like tau and amyloid precursor protein (APP) influencing pathological aggregation processes. Understanding the role of HCE in these disorders opens potential avenues for therapeutic targeting to mitigate protein aggregation and fibrous plaques characteristic of neurodegeneration.