Abcam, ab251349, Anti-PCGF5 antibody [EP19476] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PCGF5 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP19476,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251349 is the carrier-free version of
ab201511
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PCGF5 also known as Polycomb group RING finger protein 5 functions as an essential component of the cellular machinery involved in transcriptional repression. This protein has a molecular mass of approximately 47 kDa. PCGF5 is widely expressed in various tissues suggesting a role in multiple cellular contexts. It associates with other proteins as part of the Polycomb Repressive Complex which modulates gene expression by altering chromatin structure.
Biological function summary
PCGF5 participates extensively in the regulation of gene expression by participating in the Polycomb Repressive Complex 1 (PRC1). It contributes to maintaining transcriptional silencing of target genes which is important for processes like development and cell differentiation. The protein acts in concert with other members of the Polycomb group working together to modify histone proteins and maintain genes in a repressed state through chromatin remodeling.
Pathways
PCGF5 plays a significant role within the Wnt signaling pathway and the Notch signaling pathway. These pathways are integral to cellular processes such as proliferation and differentiation. PCGF5 interacts with proteins such as Bmi1 and RING1B in the PRC1 complex influencing these important signaling routes. Its activity ensures proper regulation and maintenance of gene expression patterns necessary for cellular homeostasis.
PCGF5 has implications in cancer and neurodevelopmental disorders. Its dysregulation can lead to aberrant expression of genes critical in cell cycle control and differentiation contributing to tumorigenesis. In some cases altered function or expression of PCGF5 associates with proteins like EZH2 in the context of cancer. Furthermore disruptions in its activity may relate to defects in neural development showcasing its broader relevance in human health.