Abcam, ab251367, Anti-iNOS+nNOS antibody [EP16634] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal iNOS antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Recombinant full length protein - Mouse, Mouse, Human, Recombinant full length protein - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP16634,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:I-ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:We were unable to detect a positive signal in inflammation‑induced THP‑1 cells. According to published studies (PMID:33553532, PMID: 24477906), human macrophages produce very little detectable iNOS and NO in response to inflammatory stimuli due to epigenetic regulation around the NOS2 transcription start site.

Product details:
ab251367 is the carrier-free version of
ab202417
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The iNOS and nNOS also called inducible nitric oxide synthase and neuronal nitric oxide synthase are enzymes that produce nitric oxide (NO) a signaling molecule involved in various physiological processes. iNOS and nNOS differ in their expression and regulation. iNOS is found in various tissues and is induced under inflammatory conditions while nNOS is constitutively expressed in neurons. The molecular weights of iNOS and nNOS are approximately 131 kDa and 160 kDa respectively. Both isoforms exist as homodimers facilitating the conversion of L-arginine to L-citrulline and nitric oxide.
Biological function summary
Nitric oxide produced by these synthases acts as a neurotransmitter and an immune mediator. In neurons nNOS-derived NO modulates synaptic plasticity and cognitive function significantly affecting processes such as memory and learning. iNOS-derived NO has roles in host defense and inflammation. During immune responses iNOS expression increases and produces NO to help in pathogen destruction. Both enzymes are part of multi-protein complexes that include cofactors like NADPH FAD FMN and tetrahydrobiopterin all necessary for catalytic activity.
Pathways
The nitric oxide produced by iNOS and nNOS participates in the nitric oxide signaling pathway and the cyclic GMP (cGMP) pathway. Nitric oxide activates soluble guanylate cyclase in the cGMP pathway which in turn regulates various physiological processes including vasodilation. nNOS is related to synaptophysin through neuronal pathways affecting neurotransmitter release and neuronal excitability. iNOS is associated with cytokines like TNF-α and IFN-γ in inflammatory signaling pathways having roles in cell death and immune signaling.
Nitric oxide dysregulation by iNOS and nNOS impacts numerous conditions including neurodegenerative diseases and chronic inflammation. In neurodegenerative diseases like Alzheimer's excessive NO production by nNOS can lead to neurotoxicity. Inflammatory disorders such as rheumatoid arthritis involve increased iNOS expression resulting in high levels of nitric oxide and consequent tissue damage. Both iNOS and nNOS through NO production interact with proteins such as amyloid-beta in Alzheimer’s and with various cytokines in inflammatory disorders.