Abcam, ab251379, Anti-NDUFB3 antibody [EPR15571] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NDUFB3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15571,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IHC-P, WB, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251379 is the carrier-free version of
ab202585
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein NDUFB3 also known as NADH:ubiquinone oxidoreductase subunit B3 plays a significant role in cellular respiration. It is a component of the mitochondrial complex I where it participates in the electron transport chain. NDUFB3 is a small protein with a molecular weight of approximately 12 kDa. It is expressed in various human tissues notably in high-energy-demanding organs such as the heart brain and skeletal muscles where mitochondria are abundant.
Biological function summary
NDUFB3 contributes to the transfer of electrons from NADH to ubiquinone an essential step in oxidative phosphorylation. This protein is part of the larger NADH dehydrogenase complex also called complex I which consists of 45 protein subunits. Complex I is the largest enzyme complex in the electron transport chain and serves a fundamental role in ATP production by establishing a proton gradient across the inner mitochondrial membrane.
Pathways
NDUFB3 significantly affects mitochondrial energy metabolism. It is an integral component of the electron transport chain. Through this it participates in pathways such as oxidative phosphorylation and cellular respiration. NDUFB3 associates with other proteins subunits of complex I such as NDUFS2 for electron transport which ultimately powers ATP synthesis by ATP synthase.
Disruptions in NDUFB3 function can contribute to mitochondrial diseases particularly those related to energy metabolism. Mutations in this protein have been linked to Leigh syndrome a severe neurological disorder. Moreover the dysfunction of complex I which includes NDUFB3 has connections to Parkinson's disease highlighting the importance of this protein in neurodegenerative conditions.