Abcam, ab251444, Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Ionotropic Glutamate receptor 2 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Rat, Mouse, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18115,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251444 is the carrier-free version of
ab206293
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ionotropic glutamate receptor 2 and 3 also known as GluR2 and GluR3 are subunits of AMPA receptors which are ion channels facilitating synaptic transmission. These receptors bind glutamate an important neurotransmitter and become permeable to cations leading to rapid excitatory synaptic signaling. The combined molecular masses of GluR2 and GluR3 subunits are approximately 200 kDa. They are predominantly expressed in the central nervous system particularly in the cortex hippocampus and cerebellum where they contribute to fast synaptic transmission and plasticity.
Biological function summary
These receptors play a role in regulating synaptic strength and neuronal communication. They are part of AMPA receptor complexes vital for modulating synaptic plasticity that underlies learning and memory. GluR2 contains a Q/R site near pore region controlling calcium permeability and channel conductance. This editing is necessary to prevent excessive calcium entry ensuring regulated neuronal activity and maintaining neuroprotective functions.
Pathways
AMPA receptors including GluR2 and GluR3 integrate into the glutamatergic system and are significantly involved in the long-term potentiation (LTP) pathway essential for synaptic plasticity. They interact with proteins like NMDA receptors which help the initiation phase of LTP. They also play a role in excitotoxicity pathways where excessive glutamate can lead to neuronal damage. This interaction with glutamate saves as a bridge between normal synaptic activity and dysregulated excitatory signals.
Ionotropic glutamate receptor dysregulation associates with neurodegenerative diseases like Alzheimer’s disease and seizure disorders such as epilepsy. In Alzheimer's altered receptor trafficking leads to impaired synaptic function with amyloid-beta interacting with receptor systems including NMDA receptors. For epilepsy aberrant glutamate signaling through AMPA receptor subunits like GluR2 and GluR3 is a contributing factor to excitotoxicity and seizure pathogenesis suggesting potential therapeutic targets within receptor signaling pathways.