Abcam, ab251495, Anti-1-methyladenosine (m1A) antibody [EPR-19836-208] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal 1-methyladenosine (m1A) antibody. Carrier free. Suitable for IP, ELISA, Dot and reacts with Modified Nucleic Acid, Modified Amino Acid samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR-19836-208,
Isotype:IgG,
Carrier free:Yes,
Applications:ELISA, Dot, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Has been developed to discriminate between the modified base 1-methyladenosine (m1A) and the unmodified counterpart Adenosine (A).

Product details:
ab251495 is the carrier-free version of
ab208196
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
1-Methyladenosine (m1A) is a modified nucleoside present in RNA molecules. It serves as an indicator of methylation which is a critical post-transcriptional modification. In terms of alternate names it can also be called m1A and m-1A. The mass of 1-methyladenosine is approximately 267.26 g/mol. This modification is abundant in various types of RNA including tRNA and rRNA where it is expressed in several cellular compartments. It can be detected using laboratory techniques such as dot blotting and understanding its presence can aid in identifying RNA methylation states.
Biological function summary
1-methyladenosine plays a significant role in the stability and function of tRNA and rRNA molecules. This modification influences the proper folding and functionality of these RNA types ensuring accurate translation processes within the ribosome. 1-methyladenosine is part of complex interplay with different enzymes including methyltransferases that catalyze the addition of methyl groups. These enzymes are integral in maintaining the methylation pattern critical for cell homeostasis and function.
Pathways
RNA methylation is closely involved in gene expression regulations and protein synthesis pathways. m1A is important in pathways like the translation initiation process which requires precise RNA modifications for ribosomal function. Proteins such as methyltransferases are linked with m1A through these pathways helping transfer methyl groups to RNA molecules. This process affects downstream biological effects linked to cellular growth and replication.
Aberrations in 1-methyladenosine levels are associated with specific health conditions. Disruptions in m1A levels can relate to certain cancers where methylation patterns are often dysregulated. Connections exist between m1A and proteins like dyskerin which play roles in pseudouridylation and are implicated in cancer progression. Another disorder related to altered m1A is metabolic syndrome where imbalanced RNA modifications can impact metabolic pathways. These associations offer insights into potential therapeutic targets involving RNA methylation.