Abcam, ab251529, Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal N4-methylcytidine (m4C) antibody. Carrier free. Suitable for IP, ELISA and reacts with Modified Nucleic Acid samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR -19850-108,
Isotype:IgG,
Carrier free:Yes,
Applications:ELISA, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Has been developed to discriminate between the modified base N4-methylcytidine (m4C) and the unmodified counterpart Cytidine (C).

Product details:
ab251529 is the carrier-free version of
ab211494
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N4-methylcytidine (m4C) is a modified nucleoside found in RNA specifically involved in post-transcriptional modifications. It is sometimes referred to as methylcytidine and its mass is approximately 257.23 Da. You can find m4C expressed in various prokaryotic and eukaryotic systems suggesting its broad role in molecular biology. Notably the methylation process leading to m4C is facilitated by specific methyltransferase enzymes. Because of this enzymatic activity the nucleoside exhibits diverse mechanical functions that influence RNA stability and translation efficiency.
Biological function summary
M4C plays a role in the regulation of gene expression. It is normally observed as part of the ribosomal RNA in both bacterial and mammalian cells indicating its conserved function across species. The methylation at the cytidine position in RNA helps stabilize the RNA structure which contributes to the control of protein synthesis. m4C does not directly form part of any known complex but the modifications it undergoes indirectly affect ribosomal complexes and their function.
Pathways
The modifications introduced by m4C are fundamental to the mRNA translation pathway and RNA processing pathway. The methylated form interacts with ribosomal proteins influencing translational fidelity and efficiency. m4C also associates with RNA polymerase proteins due to its participation in message stability. These interactions position m4C as a pivotal contributor in maintaining cellular homeostasis by regulating protein synthesis through these essential pathways.
M4C modifications relate to certain types of cancers and neurological disorders. Abnormal m4C levels can disrupt normal gene expression patterns contributing to oncogenesis. Furthermore its association with ribosomal processing implicates it in neurodegenerative diseases where protein misfolding is a characteristic feature. Proteins like ribosomal protein S5 and transcription factor E2F1 have connections to m4C's altered functions in these conditions revealing the complex role m4C plays in disease etiology. Understanding m4C's involvement might help in developing targeted therapies for conditions linked to translation deregulation.