Abcam, ab251538, Anti-HNF-1B antibody [EPR18644-37] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HNF-1B antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18644-37,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognizes a different epitope of HNF-1B compared to ab213149.

Product details:
ab251538 is the carrier-free version of
ab213150
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HNF-1B also known as hepatocyte nuclear factor 1-beta is a transcription factor with a molecular mass of approximately 56 kDa. This protein regulates gene expression by binding to specific DNA sequences influencing the transcriptional activity of target genes. HNF-1B is highly expressed in tissues such as the kidney liver pancreas and lung. It plays a critical role in the development and function of these organs exerting control over the expression of genes involved in their respective physiological processes.
Biological function summary
HNF-1B functions are integral to organ development and tissue-specific gene expression. It is part of the HNF family of transcription factors and forms complexes with other members like HNF-1A. HNF-1B regulates genes that are essential for epithelial cell differentiation and organ morphogenesis. This regulation ensures proper functioning of multiple organs highlighting its significance in maintaining organ health and homeostasis.
Pathways
HNF-1B is deeply integrated into the Wnt signaling pathway and renal-specific pathways. It modulates the expression of key components within these pathways such as promoting renal tubule development and function. HNF-1B interacts with other proteins like TCF7L2 and β-catenin facilitating its role in Wnt signaling. These interactions guide precise cellular responses allowing HNF-1B to coordinate complex biological processes like organogenesis and cellular differentiation.
Mutations in the gene encoding HNF-1B contribute to renal cysts and diabetes syndrome (RCAD) and maturity-onset diabetes of the young type 5 (MODY5). These genetic variations disrupt HNF-1B function leading to impaired organ development and function. In RCAD HNF-1B abnormalities link closely to kidney malformations and diabetic symptoms interacting with genes like PAX2 involved in kidney formation highlighting the molecular complexity associated with these conditions.