Abcam, ab251562, Anti-HDAC1+HDAC2 antibody [EPR20327] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HDAC1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20327,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, WB, IP, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251562 is the carrier-free version of
ab219054
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDAC1 and HDAC2 also known as histone deacetylases 1 and 2 are enzyme molecules with masses of approximately 55 kDa each. Mechanically HDAC1 and HDAC2 remove acetyl groups from histone proteins which affects chromatin structure and transcription regulation. These proteins exert their function within the nucleus of eukaryotic cells. They are expressed in various tissues but have significant levels in the brain heart and muscles.
Biological function summary
HDAC1 and HDAC2 function together as part of larger multiprotein complexes such as the Sin3 and NuRD complexes. These enzymes play key roles in the repression of gene expression. By altering the acetylation status of histones they contribute to chromatin condensation silencing certain gene areas. Their activity is necessary for proper development cell proliferation and differentiation. Scientists have observed that HDACs help maintain stem cell pluripotency and regulate cell cycle progression.
Pathways
HDAC1 and HDAC2 are integral to signaling pathways involved in DNA repair and cell cycle regulation. Specifically they impact the Notch and Wnt pathways which are essential for cell communication and cellular events like apoptosis and differentiation. Notably HDAC1 and HDAC2 interact with proteins such as Rb (retinoblastoma protein) and E2F transcription factors influencing cellular progression through the cell cycle and thereby affecting cell fate decisions.
HDAC1 and HDAC2 have links to cancer and neurological disorders. Their dysregulation can result in aberrant gene expression profiles contributing to tumorigenesis and cancer progression. For instance overexpression of HDACs in various cancers has been reported suggesting that these enzymes facilitate oncogenic processes. HDAC1 and HDAC2 also connect to neurodegenerative diseases by influencing proteins like those involved in Alzheimer's disease; their role in chromatin remodeling and gene expression impacts neuronal cell survival and function.