Abcam, ab251567, Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ZO1 tight junction protein antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19945-224,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251567 is the carrier-free version of
ab221546
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ZO1 tight junction protein also known as TJP1 is a critical component of tight junctions in epithelial and endothelial cells. It is a 225 kDa protein that plays a mechanical role in stabilizing cell-cell junctions by linking the actin cytoskeleton to tight junction membrane proteins. ZO1 is expressed in various tissues including the liver kidney and brain where it is essential for maintaining cell polarity and permeability barriers. It acts as a scaffold facilitating the organization of other tight junction proteins.
Biological function summary
ZO1 tight junction protein maintains cellular barriers and regulates paracellular permeability. This protein forms part of the tight junction complex with other proteins like occludin and claudins contributing to cellular integrity. ZO1's presence at tight junctions helps control the passage of ions and molecules between cells therefore ensuring selective permeability while protecting tissue function. Its ability to interact with multiple proteins enables it to coordinate complex signaling mechanisms that are important for cell adhesion and communication.
Pathways
The tight junction protein ZO1 participates in important signaling pathways like the epithelial barrier formation pathway and the actin cytoskeleton regulation pathway. It interacts with proteins like ZO2 and ZO3 which are critical for enhancing barrier function and cytoskeletal dynamics. By participating in these pathways ZO1 helps mediate both the structural and signaling functions necessary for maintaining tissue homeostasis and responding to cellular stress or injury.
ZO1 protein plays a role in conditions such as inflammatory bowel disease (IBD) and cancer. In IBD the disruption of ZO1 leads to compromised intestinal barriers contributing to disease pathogenesis. The loss or mislocalization of ZO1 has been associated with tumorigenesis in cancers where its interaction with other junctional proteins like claudins could influence cancer cell invasiveness. Understanding ZO1's involvement in these disorders provides insights into potential therapeutic targets for improving epithelial barrier function.