Abcam, ab251591, Anti-SLC1A5/ASCT2 antibody [CAL33] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SLC1A5/ASCT2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:CAL33,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, ICC/IF, Flow Cyt (Intra), IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251591 is the carrier-free version of
ab237704
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purity is greater than 99%., Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SLC1A5 protein also known as ASCT2 is a sodium-dependent neutral amino acid transporter with a molecular mass of approximately 56 kDa. It plays an important role in the transport of amino acids such as glutamine across the plasma membrane. This transporter is widely expressed in multiple tissues including the liver brain and kidneys. ASCT2 is integral in maintaining amino acid balance within cells facilitating nutrient uptake essential for cellular metabolism and function.
Biological function summary
The SLC1A5 protein contributes to cellular processes by facilitating the uptake of neutral amino acids. ASCT2 operates as a part of a complex of transporters that ensure the supply of critical nutrients within cells impacting metabolic processes like protein synthesis and cell growth. Its function supports the dynamic needs of rapidly growing tissues and is especially active in conditions demanding high amino acid turnover.
Pathways
The activity of SLC1A5/ASCT2 is important in amino acid transport pathways particularly influencing the mTOR signaling which plays a significant role in cell growth and metabolic regulation. SLC1A5/ASCT2 interaction with proteins like LAT1 strengthens its involvement in these pathways. These interactions outline a network of nutrient sensing and metabolic control important for cellular proliferation and homeostasis.
SLC1A5/ASCT2 shows a significant connection to cancer as many cancer cells exploit its role in glutamine transport to fuel their rapid growth and survival. Additionally its involvement is noted in metabolic disorders where dysregulated amino acid transport can lead to imbalances in cellular metabolism. SLC1A5/ASCT2 has potential interactions with proteins like glutaminase which are often upregulated in oncogenic pathways highlighting its importance as a target for therapeutic interventions.