Abcam, ab251741, Anti-MPRIP antibody

Size: 100µL
Rabbit Polyclonal MPRIP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MPRIP aa 850 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MPRIP aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q6WCQ1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MPRIP also known as Myosin Phosphatase Rho Interacting Protein or p116Rip is an important player in the regulation of the actin cytoskeleton. This protein weighs approximately 116 kDa and is expressed in a variety of tissues including the brain heart and testis. It interacts with the regulatory subunit of myosin phosphatase influencing actin filament dynamics by modulating the interaction between actin and myosin.
Biological function summary
MPRIP serves a critical role in the modulation of actin cytoskeleton rearrangement. It is part of a multi-protein complex that includes myosin phosphatase which is essential for dephosphorylation of myosin light chains. By binding directly to the myosin phosphatase complex MPRIP ensures proper regulation of actin-myosin interactions which are integral to cellular processes such as migration division and shape maintenance.
Pathways
MPRIP plays an important role within the RhoA signaling pathway and the regulation of cytoskeletal rearrangements. It facilitates the interaction between RhoA and myosin phosphatase leading to the deactivation of contractility. Another important protein involved is ROCK as MPRIP counteracts ROCK-induced phosphorylation of myosin light chain therefore influencing cell contractility and motility.
MPRIP dysregulation has implications in cardiovascular diseases and cancer. Its connection with proteins like ROCK and RhoA highlights its role in these pathologies. In cardiovascular diseases improper MPRIP function can lead to aberrant vascular smooth muscle contraction while in cancer altered MPRIP activity may contribute to changes in cell migration and invasion affecting cancer progression and metastasis.