Abcam, ab251886, Anti-NNT1 antibody

Size: 100µL
Rabbit Polyclonal NNT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CLCF1 aa 1-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human CLCF1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.Q9UBD9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein 'NNT1' also known as nicotinamide nucleotide transhydrogenase is a mitochondrial enzyme with a mass of approximately 114 kDa. It is expressed in various tissues with high concentrations in the heart liver and kidney. Mechanically NNT1 is a membrane-bound enzyme that acts to transfer hydride ions between NADH and NADP+ facilitating the production of NADPH within the mitochondria. This process is important for maintaining cellular redox balance and supporting antioxidant defense systems.
Biological function summary
The function of maintaining NADPH levels makes NNT1 an important player in protecting cells from oxidative damage. It functions as part of the inner mitochondrial membrane and is not generally considered to be part of a larger complex in its basic form. By perpetuating a pool of available NADPH NNT1 aids cellular processes such as lipid synthesis and detoxification.
Pathways
The activity of NNT1 is integral to the maintenance of mitochondrial homeostasis particularly the oxidative phosphorylation pathway. It has associations with the glutathione antioxidant pathway as it contributes to the regeneration of reduced glutathione through provision of NADPH. The pathways involving NNT1 connect it to proteins such as glutathione reductase and those involved in the electron transport chain.
NNT1 has connections to metabolic disorders and conditions related to oxidative stress. Mutations in the NNT1 gene can be linked to familial glucocorticoid deficiency a condition affecting adrenal gland function. This protein is also related to oxidative stress which can connect it to neurodegenerative disorders with ties to proteins involved in mitochondrial dysfunction and stress responses.