Abcam, ab251967, Anti-PADI1 / PAD1 antibody

Size: 100µL
Rabbit Polyclonal PADI1 / PAD1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PADI1 aa 450-550.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PADI1 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.Q9ULC6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PADI1 also known as PAD1 or PADIone is a protein of approximately 74 kDa that belongs to the peptidylarginine deiminase family. It catalyzes the conversion of arginine residues into citrulline in a calcium-dependent manner a process known as citrullination or deimination. The PADI1 expression is mainly in the epidermis hair follicles and other keratinizing tissues. The enzyme requires at least one Ca2+ ion for its activity and often associates with the nucleus and cytoplasm within cells.
Biological function summary
PADI1 plays an important role in modulating structural changes in proteins involved in skin differentiation and hair formation. It is not known to form part of a larger protein complex but instead acts as a standalone enzyme in multiple cellular processes. The citrullination activity of PADI1 significantly affects the structural proteins like keratin and filaggrin influencing keratinocyte terminal differentiation and barrier function of the skin.
Pathways
PADI1 is instrumental in the formation of the epidermal barrier by contributing to the terminal differentiation pathway of keratinocytes. This pathway ensures the proper development and maintenance of the skin layers. It associates with proteins such as keratin and filaggrin during this process. Furthermore PADI1 also links to pathways involved in the regulation of intracellular protein modification impacting the overall protein structure and function in tissues.
PADI1 has been linked to psoriasis and skin cancers. In psoriasis the aberrant expression or regulation of PADI1 can lead to improper protein citrullination affecting skin barrier function and immune responses. Moreover dysregulated activity of PADI1 connects to skin cancer progression through its impact on keratinocyte proliferation and differentiation. Research has shown interactions with other proteins like keratin as part of these disease processes relevant to its role in skin pathology.