Abcam, ab252215, Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128]

Size: 20µL / 100µL / 1mL
Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (ab252215) is a rabbit monoclonal antibody detecting N4-acetylcytidine (ac4C) in Dot Blot . - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPRNCI-184-128,
Isotype:IgG,
Carrier free:No,
Applications:DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (ab252215) is a rabbit recombinant monoclonal antibody and is validated for use in Dot Blot.
Trusted by the scientific community
Anti-N4-acetylcytidine (ac4C) [EPRNCI-184-128] (ab252215) was first used in a scientific publication in 2019 and has been cited over 10 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [EPRNCI-184-128] also available for your convenience: ab252215, Carrier free -
ab253039
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N4-acetylcytidine (ac4C) a modified nucleoside occurs in various types of RNA like tRNA rRNA and mRNA. The molecular mass of ac4C roughly totals 255 daltons. It is expressed ubiquitously across many organisms including humans yeast and bacteria. This modification also referred to sometimes as an 'RNA acetylation mark' contributes to the stability and codon-anticodon interactions in tRNA with an important role in maintaining the structural integrity of rRNA components.
Biological function summary
N4-acetylcytidine acts to enhance translation fidelity which is essential for accurate gene expression. It ensures the stabilization of RNA structures and impacts the efficiency of translation. This modification often involves an enzyme complex including acetyltransferases which work by transferring the acetyl group from acetyl-CoA to the cytidine residue. Proper functioning and balance of ac4C modifications in RNA depend on these enzymes ensuring that the RNA molecule remains functional and effective in protein synthesis.
Pathways
N4-acetylcytidine takes part in translation and RNA processing pathways. It works alongside RNA polymerase complexes and other enzymes that modify RNA. The modification of ac4C enhances the initiation and elongation phases of translation. Within these pathways it interacts with proteins such as RNA acetylating enzymes and ribosomal proteins to fine-tune these processes ensuring that RNA synthesis and subsequent protein production are precise and efficient.
N4-acetylcytidine shows links to conditions related to translation errors and cellular stress responses. Aberrant levels of ac4C have connections to cancer wherein dysregulated translation and misfolded protein formation occur. Defective ac4C modification might lead to neurodegeneration where accurate protein synthesis is compromised. Proteins like NAT10 a known RNA acetyltransferase interact with ac4C in these pathological contexts indicating the importance of maintaining balanced ac4C levels for cellular health and disease prevention.